Contact
schliessen

Filtern

 

Bibliotheken

Logo der Bibliothek

Siegel TU Braunschweig Universitätsbibliothek Braunschweig
Interlibrary loan limitations and delayed search index updates: More information can be found in our Blog (in German).
You do not seem to be within the network of Braunschweig University.
As student, researcher or staff member of Braunschweig University you can use the VPN service to gain access to electronic publications.
Alternatively, you can use your university username and password via Shibboleth to gain access to electronic publications with certain publishers. You can find more details in our Blog (in German).

Expression of parathyroid hormone-related protein during immortalization of human peripheral blood mononuclear cells by HTLV-1: Implications for transformation

Abstract Background Adult T-cell leukemia/lymphoma (ATLL) is initiated by infection with human T-lymphotropic virus type-1 (HTLV-1); however, additional host factors are also required for T-cell transformation and development of ATLL. The HTLV-1 Tax protein plays an important role in the transformat... Full description

Main Author: Nadella Kiran S
Contributors: Thudi Nanda K | Author
Dirksen Wessel P | Author
Shu Sherry T | Author
Nadella Murali VP | Author
Fernandez Soledad A | Author
Lairmore Michael D | Author
Green Patrick L | Author
Rosol Thomas J | Author
Contained in: Retrovirology (01.06.2008)
Journal Title: Retrovirology 01.06.2008
Fulltext access: Fulltext access (direct link - free access) 10.1186/1742-4690-5-46
Links: Additional Link (dx.doi.org)
Additional Link (doaj.org)
Additional Link (www.retrovirology.com)
Fulltext access (doaj.org)
ISSN: 1742-4690
DOI: 10.1186/1742-4690-5-46
Language: English
Physical Description: Online-Ressource
ID (e.g. DOI, URN): 10.1186/1742-4690-5-46
PPN (Catalogue-ID): DOAJ010254803
more publication details ...

Associated Publications/Volumes

  • Associated records are being queried...
more (+)
Internes Format
LEADER 03684nma a2200373 c 4500
001 DOAJ010254803
003 DE-601
005 20190329101322.0
007 cr uuu---uuuuu
008 171225s2008 000 0 eng d
024 7 |a 10.1186/1742-4690-5-46  |2 doi 
035 |a (DE-599)DOAJc56d3e8a89874a03ac7eb9ca4531df76 
040 |b ger  |c GBVCP 
041 0 |a eng 
100 0 |a Nadella Kiran S  |e verfasserin  |4 aut 
245 1 0 |a Expression of parathyroid hormone-related protein during immortalization of human peripheral blood mononuclear cells by HTLV-1: Implications for transformation  |h Elektronische Ressource 
300 |a Online-Ressource 
520 |a Abstract Background Adult T-cell leukemia/lymphoma (ATLL) is initiated by infection with human T-lymphotropic virus type-1 (HTLV-1); however, additional host factors are also required for T-cell transformation and development of ATLL. The HTLV-1 Tax protein plays an important role in the transformation of T-cells although the exact mechanisms remain unclear. Parathyroid hormone-related protein (PTHrP) plays an important role in the pathogenesis of humoral hypercalcemia of malignancy (HHM) that occurs in the majority of ATLL patients. However, PTHrP is also up-regulated in HTLV-1-carriers and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients without hypercalcemia, indicating that PTHrP is expressed before transformation of T-cells. The expression of PTHrP and the PTH/PTHrP receptor during immortalization or transformation of lymphocytes by HTLV-1 has not been investigated. Results We report that PTHrP was up-regulated during immortalization of lymphocytes from peripheral blood mononuclear cells by HTLV-1 infection in long-term co-culture assays. There was preferential utilization of the PTHrP-P2 promoter in the immortalized cells compared to the HTLV-1-transformed MT-2 cells. PTHrP expression did not correlate temporally with expression of HTLV-1 tax. HTLV-1 infection up-regulated the PTHrP receptor (PTH1R) in lymphocytes indicating a potential autocrine role for PTHrP. Furthermore, co-transfection of HTLV-1 expression plasmids and PTHrP P2/P3-promoter luciferase reporter plasmids demonstrated that HTLV-1 up-regulated PTHrP expression only mildly, indicating that other cellular factors and/or events are required for the very high PTHrP expression observed in ATLL cells. We also report that macrophage inflammatory protein-1α (MIP-1α), a cellular gene known to play an important role in the pathogenesis of HHM in ATLL patients, was highly expressed during early HTLV-1 infection indicating that, unlike PTHrP, its expression was enhanced due to activation of lymphocytes by HTLV-1 infection. Conclusion These data demonstrate that PTHrP and its receptor are up-regulated specifically during immortalization of T-lymphocytes by HTLV-1 infection and may facilitate the transformation process. 
700 0 |a Thudi Nanda K  |e verfasserin  |4 aut 
700 0 |a Dirksen Wessel P  |e verfasserin  |4 aut 
700 0 |a Shu Sherry T  |e verfasserin  |4 aut 
700 0 |a Nadella Murali VP  |e verfasserin  |4 aut 
700 0 |a Fernandez Soledad A  |e verfasserin  |4 aut 
700 0 |a Lairmore Michael D  |e verfasserin  |4 aut 
700 0 |a Green Patrick L  |e verfasserin  |4 aut 
700 0 |a Rosol Thomas J  |e verfasserin  |4 aut 
773 0 8 |i In  |t Retrovirology  |g  (01.06.2008)  |w (DE-601)DOAJ000017043  |x 1742-4690 
856 4 0 |u http://dx.doi.org/10.1186/1742-4690-5-46 
856 4 0 |y DOAJ  |u https://doaj.org/article/c56d3e8a89874a03ac7eb9ca4531df76 
856 4 0 |u http://www.retrovirology.com/content/5/1/46 
856 4 0 |u https://doaj.org/toc/1742-4690 
912 |a GBV_DOAJ 
951 |a AR 
952 |j 2008  |b 01  |c 06