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Functionalization of silicone rubber for the covalent immobilization of fibronectin : Funktionalisierung von Silikongummi für die kovalente Immobilisierung von Fibronectin

Surface modification techniques were employed in order to provide functionalized silicone rubber with enhanced cytocompatibility. Acrylic acid (AAc), methacrylic acid (MAAc) and glycidylmethacrylate (GMA) were graft-co-polymerized onto the surface of silicone induced by an argon plasma and thermal i... Full description

Contained in: Journal of Materials Science - Materials in Medicine Vol. 12, No. 2 (2001), p. 111-119
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Links: Additional Link (dx.doi.org)
ISSN: 1573-4838
Additional Keywords: ACRYLSAEURE
ADHAESION
GEWEBEVERTRAEGLICHKEIT
GLYCIDYLMETHACRYLSAEUREESTER
INFRAROTSPEKTROMETRIE
METHACRYLSAEURE
OBERFLAECHENANALYSE
OBERFLAECHENBEHANDLUNG
PFROPFCOPOLYMERISATION
RASTERELEKTRONENMIKROSKOPIE
ROENTGENPHOTOELEKTRONENSPEKTROSKOPIE
SCHICHT:BELAG
SILICON:POLYMER
SILICONKAUTSCHUK
DOI: 10.1023/A:1008938525489
Notes: Copyright: Metadaten: TEMA, Copyright WTI-Frankfurt eG
Copyright: (C) Alle Rechte beim Herausgeber
Physical Description: 9 Seiten, 9 Bilder, 1 Tabelle, 24 Quellen
ID (e.g. DOI, URN): 10.1023/A:1008938525489
PPN (Catalogue-ID): WTI037980882
Note: WTI TEMA DB
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520 |a Surface modification techniques were employed in order to provide functionalized silicone rubber with enhanced cytocompatibility. Acrylic acid (AAc), methacrylic acid (MAAc) and glycidylmethacrylate (GMA) were graft-co-polymerized onto the surface of silicone induced by an argon plasma and thermal initiation. The polymerizations were carried out in solution, in the case of acrylic acid a vapor phase graft-co-polymerization subsequent to argon plasma activation was carried out as well. Human fibronectin (hFn), which acts as a cell adhesion mediator for fibroblasts, was immobilized by making use of the generated carboxylic or epoxy groups, respectively. Surface analysis was accomplished by means of X-ray photoelectron spectroscopy (XPS), infrared spectroscopy in attenuated total reflection mode (IR-ATR), scanning electron microscopy (SEM), atomic force microscopy (AFM) and dynamic contact angle measurements using the Wilhelmy-plate method. The amount of immobilized active hFn was semiquantified by enzyme-Iinked immunosorbent assay (ELISA) using a structure-specific antibody against the cell-binding domain of hFn. In vitro testing showed a remarkable difference between surfaces exposing adsorbed-only and surfaces with covalently immobilized hFn. 
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